Can you send us your Skyline document?

In Skyline, you can use the menu item:
File > Share > (complete)
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

You can upload that .zip file here:
https://skyline.ms/files.url

-- Nick

done file name is 20180910_Gavin_FASTA_link_0_99conf_lib.sky

That view works for me (see attached screenshot).

I think maybe you have chosen something on the right-click menu which is preventing any points from being displayed.

For instance, if you have chosen "Group By > BioReplicate" then it will say "Not enough data" because, since the each replicate has a different value for BioReplicate in the dataset, there is nothing for Skyline to calculate CVs from.

If this still isn't working for you, can you attach a screenshot of what you're seeing?

Must be something else since I've tried group by ""all replicates" and ""condition"" but still same message "not enough data points"" see attached

I am going to guess it is right-click > Transitions > Precursors is what you have set, and you will see the histogram if you use right-click > Transitions > Products. I just made the switch on your document, and it does have this effect.

This has bitten me before, and I personally think we need to fix this to not even offer this option in documents like yours without precursor ions.

Sorry for the confusion. Thanks for reminding me we need to fix this.

--Brendan

You are spot on! Thank you. Now that you have highlighted it seems like a very obvious thing I should have checked but completely missed it :)