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SMALL MOLECULES, M+1, easy

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SMALL MOLECULES, M+1, easy henrik molina  2018-09-07 06:03
 

Dear SkyLine (Daily) team
I am analyzing SMALL MOLECULES using high res/high mass acq MS1 data. I generally use ONLY the mono isotopic signal but will like to add M+1. I have a rather long list of molecules (200) where I would like to add M+1. Is there an easy way (not one by one) to add M+1 for all my SMALL MOLECULES?
Cheers, h
 
 
Brian Pratt responded:  2018-09-07 12:06

Hi Henrik,

If you go to the Settings>TransitionSettings>FullScan and change the MS1 Filter "Peaks:" count to 2, those isotopes should appear in the Targets tree.

Brian
 
henrik molina responded:  2018-09-07 12:38

Thank you. This is what I thought (and tried) but it is not working for me: changing [Peaks] to for example '2'. The default value is already '3'.
We input the list of targetes/molecules using 'Edit' -> 'insert' -> 'transition list': Small Molecules
h
 
Brian Pratt responded:  2018-09-07 12:49

May I see the Skyline document you're using (use File>Share>Complete, and send that to http://skyline.ms/files.url)?

Thanks

Brian
 
henrik molina responded:  2018-09-07 12:57

.sky file is uploaded. I have also uploaded a file with ''_hm' at the end. This one seems to be corrupoted. We often enough see this happen and have no idea why.
h
 
Brian Pratt responded:  2018-09-07 14:31
You just need to adjust your settings slightly: in Settings>TransitionSettings>Filter make sure the "Auto select all matching transitions" box is checked, and make sure the "Ion Types" setting contains "p" for "precursors" ("p" should really be there by default, I'll fix that). That corrupted file is very strange. May I see the transition list you started with? Thanks Brian
 
henrik molina responded:  2018-09-08 12:20
Thx! That fixed my issue! Will try to report the other problem with corrupted .sky files. h