Modification not resolved | Gereon | 2018-08-22 03:30 | |||||||||||||||||||||||||||
Dear support team, I tried to quantify a few petides with the DDA with MS1 filtering worklflow (Skyline 4.1.0.18169). Here I would like to quantify also two variable cysteine modifications (NEM and its heavy labeled variant NEMD5). As for some peptides there might be also combinations possible I tried to define both as structural (variable) modifications. The NEM modification is resolved pretty well, but the heavy one (NEMD5) is not recognized by skyline althought the corresponding peptide identification is present in library. Here, it is written that the modification (C[+130.07632] ) cannot be resolved. The theoretical shift is +130.079062. Thanks, Gereon |
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