There is a column that you can enable in the Edit>Insert>TransitionList dialog, called "Explicit Retention Time Window".

Note that if your transition list uses header column names that Skyline recognizes, you can just copy and paste right into the Targets window.

Thanks for using the Skyline support board!

Brian Pratt

Hi Brian,

Thanks for your answer. What I am asking is the input format for explicit retention time window, that is, what I enter into the cells if I want Skyline to integrate from, for example, 3.2 min to 3.9 min? I have tried "3.2-3.9", "3.2/3.9" "3.2,3.9" and none of them worked. It only takes a number. However, it doesn't seem to define any time ranges either, even if I put in both the explicit retention time and the explicit retention time window values. I have attached an example and hopefully this will help.

Best Regards

Kang-Yu

There are two columns in play there - Explicit Retention Time, and Explicit Retention Time Window.

Explicit Retention Time is the center of the RT range, Explicit Retention Time Window is the width of the range.

So for your example, RT 3.55 with a window of 0.7

But Skyline will look within that window for the best single peak rather than lump peaks together. The range is used as a peak search boundary rather than as an instruction to add up everything within the boundary.

So, not quite the answer you were hoping for, sorry.

Best,

Brian

Hi Brian,

Thanks! Now I understand how it works.

Best Regards

Kang-Yu