If any of your peptides are missing a value for a particular feature (for instance, because the peptide is not present in your spectral library), then Skyline will display the feature in gray italics, and you will not be able to use that feature in your model.
The "Feature Scores" tab in the Edit Peak Scoring dialog shows you what the values are for that feature for the peptides in your document. If the feature is available for some peptides but not others, there will be a bar in the bar graph "unknown". If you move the mouse under that "unknown", a binoculars button appears, and when you press it, the Find Results window will contain a list of all of the peptides missing that feature.
The Library Intensity Dot Product will be missing if the peptide is not present in your spectral library, or if the peptide has fewer than (I believe) 3 product ion transitions. |