Handle low quality library

support
Handle low quality library user  2018-07-31 09:01
 

Dear Skyline developers,

I find in some cases, even the library of the “best replicate” is not good enough and thus dotp values might not be accurate. If I want to replace a few low quality spectra for some peptides in a library with high quality library spectra from PeptidesAtlas etc., does Skyline support this? Can Skyline remain the old RT in this case?

Thanks,
Antony

 
 
Brendan MacLean responded:  2018-08-11 15:10

Hi Antony,
We don't provide any ability to edit spectral libraries in the Skyline UI. Your only option would be to use the SSL format to construct your own library. Then if you order that library before your current library, its values would take precedence.

https://skyline.ms/wiki/home/software/BiblioSpec/page.view?name=BiblioSpec input and output file formats

Hope this helps.

--Brendan