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Fluorine modification

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Fluorine modification nicakruh  2018-07-26 12:17
 

I am trying to monitor peptides from a recombinant protein in which a fluorinated tryptophan was incorporated. I am trying to add this modification to the peptide settings, however, the mass calculation doesn't appear to take the loss of H into account and is adding 19+ instead of 18+ to the peptide. How can this be corrected?
 
 
Nick Shulman responded:  2018-07-26 12:30
When are writing the chemical formula of the modification in the "Edit Structural Modification" dialog, the formula is allowed to have two parts separated by a minus sign, and the second part gets subtracted from the first.
So, the chemical formula a fluorine modification would be:
F - H

Skyline actually already knows about this modification. It's called "Fluoro (W)".