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Fabian responded: |
2018-09-14 05:18 |
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derock j responded: |
2019-03-14 00:44 |
Hello,
I think I have the same problem. When I use the idotp refine option, ome peaks with values below the set threshold are kept and I don't understand why.
Best regards
Jérémy
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htl responded: |
2020-05-28 14:08 |
Hi,
I have the same question. Is it possible to remove the low idotp peaks from particular replicate? "Min idotp" from Refine, Advanced remove the peptide from all replicates.
Thanks
Ho-Tak
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Nick Shulman responded: |
2020-05-28 15:11 |
When you specify something for the "Min idotp" in the Refine > Advanced dialog, Skyline deletes Precursors from the document where the average Isotope Dot Product across all the replicates is lower than that value.
On the Refine Dialog, you can also choose "Best" for "Replicate Inclusion", instead of "All". In that case, Skyline only looks at Isotope Dot Product of the replicate with the largest area.
If you would like to remove the peaks that have low Isotope Dot Products, you should use the Document Grid.
Once you have all of the rows in the Document Grid selected which you want to remove, you can use the "Actions" dropdown at the top of the Document Grid.
There's a menu item on the Actions dropdown "Remove Precursor Peaks".
-- Nick |
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