Hello,

I try to implement MSX-DIA in Orbitrap Lumos by generating isolation list from Skyline 4.1. However, I could not find a way to import them into the method. When I select DIA in Xcalibur 4.1 and checked the multiplex option, the isolation table is only readable. Do I need to choose PRM mode instead of DIA?

Another question is do you recommend overlapping isolation scheme or MSX for Orbitrap Lumos? My understanding is that orbitrap take some time for mass analysis, so it should benefit from using multiplexing (less number of scan?).

Thank you very much.