Hi Brendan,
One more question about how skyline predicts peptide retention times. I've attached a chart to highlight my question. In this scenario, the same peptide elutes at different times with considerably varying ion intensities. The user selecting the correct peaks and recalibrated the iRT database shows near perfect linear regression, however, my question pertains to the yellow prediction box. How is this time predicted? It does not appear to correlate with the SSRCalc times.
All the best and thank you for your help!
Johnny |