Dear Skyline community,

I have performed a targeted analysis of a small molecule, including 13C internal standard.

After importing the results into skyline, transitions and peak areas for the small molecule analyte and 13C standard are properly assigned and calculated (MRM, centroid, Waters TQ).

However, the Peak Area ratios of "Analyte" / "13C internal standard" are not shown and give an error (missing values) in the calibration curve. Both transitions, analyte and internal standard (=heavy)) are assigned to the same small molecule (I followed the instruction from the absolute quantification application note, for peptides it works well)

Any help is highly appreciated.

Best regards.