QC with Skyline Alone

support
QC with Skyline Alone victor nesati  2018-02-19 22:42
 
I was reading a bit about Skyline QC capabilities and looks like current workflow consists of
AutoQC Loader, Skyline processing and pushing those results to Panorama for further visualizations.
I am all for it except that we have limited access to internet on our data acquisition computer and while
first two legs of this pipeline are easily implemented, pushing to Panorama would be a bit of an issue.
If I understand correctly, Panorama has a bit more options with regards to visualization, but in principle some basic info like Peptide Areas, RT, FWHM, etc could be easily tracked in Skyline itself. So in principle upload to Panorama could be skipped, correct ?
Cheers
Victor
 
 
Brendan MacLean responded:  2018-02-20 00:37
Yes, that is true. You can set AutoQC Loader up to simply import your QC runs into a Skyline document that resides on the instrument PC. In fact, that is always how it works. If you don't give AutoQC Loader a Panorama upload URL, then it just does not upload to Panorama.

However, I will also point out that others have solved the issue with instrument PCs not connected to the Internet, but having the instrument PCs post their QC runs to an internal shared server, and then simply having an Internet-connected PC monitoring the shared server location. There is really no reason Skyline needs to be running on the instrument PCs. Though, it does have the convenient side-effect of leaving a Skyline document on the PC that you can look at, which is also all you get if you don't upload to Panorama.

So, certainly options open to you whether you want only a local Skyline document or even full Panorama QC support.

Thanks for your interest in Panorama QC and for posting to the Skyline support board.

--Brendan
 
victor nesati responded:  2018-02-20 14:51
Thanks Brendan,
Thats a good idea to do processing on data processing computers, which are outside lab, and do have connections to Internet. So this is one of potential solutions.
Alternatively do you think it is possible to get our own version of Panorama and host it on our local server ?
If there are some financial aspects involved lets discuss them.
Cheers
Victor