Thanks for reporting this bug!
Skyline is confused about what to do about the heavy precursors under the peptide when doing the "Ratio to Heavy". Instead of skipping them, Skyline ends up averaging in an invalid numeric value, which results in the behavior that you see.
As far as I can tell, this "Ratio to Heavy" never worked correctly with multiple peptide view (I don't see anything that might have changed to cause this behavior).
I will try to fix this. |