CE Optimization Observation

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CE Optimization Observation mburgess  2018-01-31
 
Hello-
I am using version 4.1.0.11714 of Skyline on a Windows 7-run PC operating a Waters Xevo TQ-S. I have utilized the CE Opt tutorial in the past and generated a series of methods with +/- 5 steps around a calculated collision energy value. The output methods from Skyline produce the proper number of transitions in the MassLynx MRM method and the data is collected normally. However, when I input the data from the runs into Skyline, I am only observing +/- 3 steps currently. Not sure if anyone else is seeing this. Am I missing a setting somewhere?
Many thanks in advance,
Mike B
 
 
mburgess responded:  2018-01-31
Hello-
It turns out if you import the data into version 4.0.9.11707, all 5 steps are observed. Seems like there's a difference between 4.1.0 and 4.0.9?
Many thanks in advance,
Mike B
 
Nick Shulman responded:  2018-01-31
Can you send us your files? I will take a look.

In Skyline you can use the menu item:
File > Share > (complete)
to create a .zip file containing your Skyline document and supporting files including spectral libraries and extracted chromatograms.

You can upload that .zip file, as well as at least one of your raw data files here:
https://skyline.ms/files.url
 
waltteri hosia responded:  2019-04-02
Hello, I delete my question from above as it was user error. Forgot to click ce optimizing button when importing. Sorry!