Dear all,
Does anyone know how to use CiRT/SiRT in Skyline to calibrate the RT variations between the selected spectrum in a merged library and the corresponding query spectrum in different runs? SpectraST converts the retention times of all identified peptides in all runs into iRT scale. Does Skyline use the same strategy, or Skyline only calibrate the RT variations between the library spectrum and the query spectrum? Can Skyline automatically extract RT values of CiRT/SiRT peptides in all runs from corresponding RAW data?
Thanks,
Antony |