Use iRT for a merged library

support
Use iRT for a merged library user  2018-01-24 20:29
 
Dear all,

Does anyone know how to use CiRT/SiRT in Skyline to calibrate the RT variations between the selected spectrum in a merged library and the corresponding query spectrum in different runs? SpectraST converts the retention times of all identified peptides in all runs into iRT scale. Does Skyline use the same strategy, or Skyline only calibrate the RT variations between the library spectrum and the query spectrum? Can Skyline automatically extract RT values of CiRT/SiRT peptides in all runs from corresponding RAW data?

Thanks,
Antony
 
 
Nick Shulman responded:  2018-01-25 13:48
I believe that BiblioSpec discards all of the iRT information when it merges libraries.

I think what you should do is combine all of the iRT databases together in Skyline.
In Skyline, in the Edit iRT Calculator dialog, you can use the "Add" button to add iRT from either another spectral library (.blib) or iRT database (.irtdb).

Does this help? I do not understand most of your question.
 
user responded:  2018-01-29 03:09
Dear Nick,

Thanks for your reply. It helps a lot.



Kind regards,
Antony