Support for mixed acquisition modes

support
Support for mixed acquisition modes Yishai  2017-11-21
 
Hello,
We have some data running targeted analysis using the Lumos where we run EThCD PRM in parrallent to other modes (DDA MS3). We also run other mixed modes on the Lumos but are unable to load the data into Skyline. Do you plan on supporting such modes?
Thanks,

Yishai
 
 
Brendan MacLean responded:  2017-12-09
Hi Yishai,
The first step would be to get us some example data with a Skyline document you wish you could load it into. You can do this by using File > Share - Complete on your document (to created a ZIP file) and then upload that and at least one raw data file to:

http://skyline.ms/files.url

And let us know it is there. Then we will have a look and assess how hard it would be to get Skyline to achieve what you are looking for. If it is not super hard, then the answer to your question would be, yes, we plan on supporting such modes. If it is super hard, then we might still "plan on supporting such modes" but the plan would take longer to execute or have an indefinite time line.

But, help us have a look at what you would like to work. It might be easy to fix.

Thanks for reporting that this is not currently working for you.

--Brendan
 
Meital Kupervaser responded:  2018-10-14
Hi Brendan,

I uploaded a BSA digest we run in PRM that includes several modes of fragmentation and is not supported by Skyline at the moment. We use this for our QC of the Lumos and fragment using CID, HCD and ETD. At the moment we can only see the MS1 signal in Skyline, but not the MS2, which we would very much like to.
The file name is FS1LC2_BSA_HCD-IT_28082018_01.raw
We'd appreciate any help with that.

Thanks,
Meital