Hi,

I have been off/on using skyline for metabolomics analysis. Recently, we took some beer samples and instead of doing the external curve, we did an internal curve. The amounts we added of S-adenosylmethionine are attached(just for reference).
The issue is…I don’t know if Skyline likes my internal curves. I did not do an external WITH the internal, so it has nothing to reference as the curve…and since the samples are not “standards,” I’m not sure what to categorize them as.
We are looking to quantify the SAM in these samples so that we can go further with some more experiments.
 
I’m not sure if it makes sense, but if you have any insight, I’d love to hear!
Thank you,

Harmonie