Results import

support
Results import sstoychev  2014-06-30 10:34
 
Hi Brendan,

When importing MRM results I only see options for replicate analysis i.e. software treats all imported runs as replicates. However, what if one wants to import non-replicates i.e. different concentrations, treatment days etc?

Stoyan
 
 
Brendan MacLean responded:  2014-06-30 10:39
Hi Stoyan,
I needed a single term to apply to everything, and I chose "replicate". In Skyline parlance, "replicate" means a set of mass spec runs (often just one run) that was used to measure your targets once. In some cases, this may take many mass spec runs (also referred to as "injections") to cover your targets just once, as in the Targeted Method Refinement tutorial where around 50 runs are used to measure each target just once. Any such collection of runs or single run which measure your target once are referred to as a "replicate", and ten sets of these would be "ten replicates", regardless of their logical organization into concentrations, subjects, time points, etc. Or whether or not any of them are actually technical replicate measurements of the same sample. Logical groupings can be made using "replicate annotations", see the Existing and Quantitative Experiments tutorial for an example (pp 33-36).

https://skyline.gs.washington.edu/labkey/_webdav/home/software/Skyline/%40files/tutorials/ExistingQuant-1_4.pdf#page=33

Sorry for the confusion. It was hard to come up with a term for this. Hope I was able to clarify enough that you can continue.

--Brendan
 
sstoychev responded:  2014-06-30 11:33
Thanks Brendan, makes sense. I suppose once runs are grouped the %CV, RT etc will be calculated based on each group as long as the "applied to" setting is selected for proteins, peptides and transitions?

Stoyan