Until I started using Skyline, new requests for protein quant and ID would require large amounts of time performing in-silico digests on prospector, copying/pasting into excel, and making my own templates for performing targeted experiments. Skyline is now a part of my regular pipeline and has allowed me to show people who might not be so mass-spec savvy, just how powerful targeted proteomics can be in their research.

I recommend it to everyone I encounter if they haven't already heard of it.

Peter Jackson
Associate Scientist - Analytical Physiology
Amyris Inc.
jackson@amyris.com