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Area Under the curve naimamuntu  2024-05-14 04:33
 

Hi,

I am currently working on a project that involves analyzing data. I am interested in the area under the curve for a product of interest. Could you please guide where to find the area under the curve for a particular precursor and one product in Skyline. I am using Skyline 21.01.0.278 for reference.
In this particular project, I am using the Chromatogram Precursor M/Z Chromatogram Product M/Z respectively, 730 and 660.25
I am sending you one skyline file so that you can see what kind of analysis we are doing.

Thank you for your attention
-Nitha
 
 
Nick Shulman responded:  2024-05-14 07:30
The usual place to see lists of numbers in Skyline is the Document Grid.
You can show the Document Grid with the menu item "View > Document Grid".
At the top of the Document Grid is a "Reports" dropdown menu item which allows you to choose different data to display. If you choose the report called "Transition Results" you will see chromatogram peak areas in the "Area" column.

The reports in the Document Grid are highly customizable.
If you would like to learn more about reports and the Document Grid you should look at the Custom Reports tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports
-- Nick