Spectral Library Building – Option: Filter for document peptides Fails | peter r mosen | 2021-06-02 09:22 | |||||||||||||||||||||
Dear Skyline team, for a MRM-assay I tried to generate a “reduced/tailored” spectral library covering only the peptides of interest using as input multiple, already existing *.blib-files (generated from DDA-data). To do so, I used the option: Filter for document peptides. The merged, resulting spectral library gets generated however it does not only contain the MS2 spectra corresponding to my peptides of interest. I.e. the “merged, reduced” spectral library (which got filtered for my 70 document peptides) comprised as many peptides as the original, full spectral library. No filtering takes place. According to Kaipo’s post (https://skyline.ms/announcements/home/support/thread.view?rowId=25366) I understand the “filter for document peptides” option, that it will consider only the activated (ticked off peptides per protein) in the target window and it will ignore deactivated peptides, right ? I am using Windows 10, Skyline 21.1.0.146 (alpha), however I had the same problem also with your latest Skyline daily version. Thank you for your help. |
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