Hi there,
May I please ask, with regards to peptide quantitation, is the sum of the peak areas of all fragment ions taken (so the total peak area of the precursor, precursor [m+1], precursor [m+2] fragment ions), or is generally the fragment ion with the greatest peak area used? May I also please ask how is the peak rank determined for each peptide/fragment ion?
Thank you so much for your help!
Cally