|peptide RT prediction does not work.||wnstjr95||2019-07-20|
I try to use iRT to predict the retention time of some peptides.
after importing MRM data, I could see the predicted and measured retention time.
I thought that I can simply get the predicted retention time since skyline uses the SSCalC.
Could you explain why it is not working? and If possilbe, let me know how to get the predicted RT without imporing MRM raw data.