Configuring TMT10Plex with Skyline

support

Hi,

 

I followed the Isotope label presentation and configured the peptide and transition setting. This product (TMT10Plex) has been used and seemed to be the same as Skyline pre-set. https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FMAN0016969_2162457_TMT10plex_UG.pdf&title=VXNlciBHdWlkZTogVE1UMTBwbGV4IE1hc3MgVGFnIExhYmVsaW5nIEtpdHMgYW5kIFJlYWdlbnRz

 

So I did the two steps that demonstrated in following documents in pages 75 and 76 of the Chemical labeling section as follow:

https://skyline.ms/_webdav/home/software/Skyline/events/2015%20Webinars/Webinar%2012/@files/Webinar%2012%20(Ludwig).pdf

  1. Added TMT6Plex (N-term) in Peptides Setting > Modifications > Structural modifications
  2. Checked all the 10 TMT options in Transition setting > Filter > Special ions

Yet, I see no TMT transitions under precursors.

 

Any suggestion as what went wrong?

I really appreciate your help.

 

Mehrnoosh