I followed the Isotope label presentation and configured the peptide and transition setting. This product (TMT10Plex) has been used and seemed to be the same as Skyline pre-set. https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2FMAN0016969_2162457_TMT10plex_UG.pdf&title=VXNlciBHdWlkZTogVE1UMTBwbGV4IE1hc3MgVGFnIExhYmVsaW5nIEtpdHMgYW5kIFJlYWdlbnRz
So I did the two steps that demonstrated in following documents in pages 75 and 76 of the Chemical labeling section as follow:
Yet, I see no TMT transitions under precursors.
Any suggestion as what went wrong?
I really appreciate your help.