Problem in analysis after saving

2020-02-18

Dear Skyline Team,

I would like to ask for your help in the following matter: I am working with proteomics, using Skyline 64-bit 4.1.0.11796. When I analyzed my data (after peak picking) I saved my Skyline datashet and closed it. After opening, it seems that the analysis (specifically my peak picking is lost/changed-please see the attached ppt). I tried to analyze the datashet in a different computer and also the option save as, instead of simple save but the same thing happens. Also, I opened some older datashets and the files seem ok (the analysis is not messed-up).

Maybe you could provide some help?

Thanks a lot,

Georgia