Unable to export methods to Analyst




A couple of weeks ago I began having problems exporting my methods into Analyst. Everything I am using is up to date (Skyline 21.2, Analyst 1.7.2...system: eksigent nLC425 with Qtrap6500). I'm not doing anything different with any of my steps in the process. It began when I tried to export a method to optimize my CE, the values did not get exported into the CE column, they were listed at the end of the name exported for the peptides in the ID column and the CE column simply had the repeating default values, but I was at least able to export some sort of a method...this was when I was working with small molecules. Last week til now I've tried to export methods and haven't been able to export any methods at all (proteomics or small molecule) and I am getting an error msg that I have never had before.

The error read..."An error occurred in attempting to export. ERROR: Unable to cast COM object of type 'System.__ComObject' to interface type 'BuildQTRAPMethod.IAcqMethodDirConfig'. This operation failed because the QueryInterface call on the COM component for the interface with IID'{.....}' failed due to the following error: Class not registered (Exception from HRESULT: 


I have been using the export transition list as a stopgap for the time being and I am able to import these into Analyst. So, I'm not sure if it is something simple that I have just overlooked, or if there is a bigger issue that I don't know about with either Skyline or Analyst or the interface between the two.


I've attached a screenshot of the error, my template method file, and an older skyline that I was able to recreate the no method export issue.

Please let me know what you think.




  Attached Files  
 2022-02-07 12_48_00-Skyline.png
 20200304_SSS bgal 8 pep lilst.sky
 20200304_SSS bgal 8 pep lilst.sky.view