Absolute Quantification

2020-05-31

Get hands-on experience using Skyline calibrated quantification to estimate the absolute molecular quantities of peptides in your experiments. (19 pages)

[download]

* - written on Skyline v1.1, updated for v1.4, updated for calibration features in v3.5, updated for v20.1

On December 1, 2015, the Skyline Team produced Webinar #12: Isotope Labeled Standards in Skyline, another great resource for this topic.
[webinar]

On April 15, 2016, the Skyline Team produced Webinar #13: Calibrated Quantification with Skyline, another great resource for this topic.
[webinar]

Also, see our paper in Nature Methods
Rapid empirical discovery of optimal peptides for targeted proteomics
[abstract]