In our first webinar in over a year, 223 participants joined Brendan MacLean (Skyline Principal Developer) and Deanna Plubell (Targeted Proteomics Researcher) on March 28, 2023 as they presented Skyline's newest tutorial, Using data independent acquisition to inform the development of triple quadrupole assays.

Deanna shared her lessons from previous work with the MacCoss lab, where she observed that DIA data is a better predictor of peptide performance in targeted triple quad assays. Deanna's presentation and tutorial walkthrough covered DIA methods to sample the proteome in a more comprehensive manner, and revealed why the type of information we can obtain from these experiments is helpful for target selection.

The webinar concluded with a great Q&A session which produced some worthwhile depth and texture to the presented material.

Q&A recap - either written response or a time stamp location of the live answer in video

-- the Skyline Team

Brendan MacLean (Principal Developer)

Presentation Slides

Deanna Plubell (Targeted Proteomics Researcher)

Presentation Slides Tutorial Notes    Files (48 MB) DIA LibA (3.0 GB)    DIA LibB (3.2 GB)    DIA LibC (2.6 GB)

Dear Skyline Users,

Join us for an all new online webinar on Tuesday, March 28, 2023 at 8:00am (Pacific Time). This webinar will explore methods for data independent acquisition to inform the development of triple quadrupole assays.

Webinar #22: Using DIA Data To Create SRM Methods

[registration closed]

• Identifying optimal peptides and SRM transitions to increase specificity and selectivity
• Understanding the benefit of using DIA data relative to DDA data for automated method building
• The importance of stable chromatography to increase overall method performance
• Key factors to use for method refinement

This webinar will include an introduction and tutorial walk-through from Brendan MacLean, Skyline Principal Developer, and a presentation from Deanna Plubell, a graduate student and course instructor in the MacCoss lab.

Join us, learn and help us to better meet your research needs using the unparalleled Skyline method development features.

--Skyline Team

Presenters

Brendan MacLean
(Principal Developer)

Deanna Plubell
(Targeted Proteomics Researcher)