Evan Hubbard currently works as a graduate student in the Julian Lab at UC Riverside, using DIA data and gas-phase radical chemistry to find new ways of detecting amino acid isomers.

Finding and Quantifying Amino Acid Isomers in Data-independent Acquisition Data to Achieve Isomer Proteomics

Some amino acids are capable of undergoing spontaneous chemical modifications to become a structural or enantiomeric isomer of the canonical residue. Confined to an individual amino acid and massless, these modifications are notoriously difficult to detect despite potentially affecting protein structure or inhibiting enzymatic digestion. Recently, we have shown that data-independent acquisition (DIA) is capable of detecting these modifications through retention time shifts of peptides containing these isomers. Such shifts produce multiple peaks in the chromatogram of a single peptide. Read More Read Less

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