All course sessions

2020 Skyline Online

SRM - PRM Session topics


Day 1 - Monday, October 5, 2020
 


  • Intro to quantitative Analysis
  • Intro to Skyline: Hands-on
  • Hands-on: Targeted MS/MS (PRM)
  • Absolute quantification and calibration curves
  • Keynote: HDL proteomics in chronic kidney disease

Day 2 - Tueday, October 6, 2020 

  • Using indexed retention time (iRT)
  • SRM/PRM instrument set up
  • Panorama, AutoQC
  • Tutorial: Manual data analysis
  • Hands-On: Grouped study data processing with Skyline
  • Review concepts

DIA - PTM Session Topics


Day 1 - Thursday October 8, 2020 
  • Welcome and intro
  • Tutorial: Analysis of DIA data in Skyline
  • DIA/PRM instrument setup
  • Peptide detection directly from DIA (Walnut)
  • (Keynote)

Day 2 - Friday October 9, 2020 

  • DIA walkthough
  • intro to PTM considerations
  • histone PTM walkthroughv
  • TBD
  • TBD

Small Molecule Session Topics


Day 1 - Thursday October 8, 2020 
10am - 6:30 pm (All times EDT)

  • Welcome and intro to Skyline for small molecules
  • Small molecule method development
  • "Futureproofing" metabolomics
  • Small molecule method transfer and quantification
  • Advanced small molecule quantification

Day 2 - Friday October 9, 2020 
9am - 6:30 pm (All times EDT)

  • SkyJam (optional) – High resolution metabolomics
  • Ion mobility mass spectrometry in proteomics and metabolomics
  • Analysis of lipidomics data in Skyline
  • System suitability tracking
  • Retention time prediction and glycan analysis
  • Gas chromatography-mass spectrometry analysis
  • The future of small molecule analysis in Skyline