Table of Contents

guest
2024-03-18
BiblioSpec Spectral Library Tools
   BlibBuild
   BlibFilter
   BlibSearch
   BlibToMS2
   LibToSqlite3
   BiblioSpec Supported Formats
BiblioSpec input and output file formats
   Example .ssl file
   Example .ms2 file
   Example .ssl file for small molecules
Download and build

BiblioSpec Spectral Library Tools


BiblioSpec is a suite of software tools for creating and searching MS/MS peptide spectrum libraries.

New in version 2.0

BiblioSpec 2.0 stores spectrum libraries as sqlite3 files. Sqlite3 is a light-weight, open-source database format which can be read and manipulated with any sqlite3 tools in addition to BiblioSpec. For more information about the library format, see the file formats page. The new format is a departure from version 1.0 which uses a unique binary format. This means that tools and libraries from the two versions are not compatible. There is, however, a conversion tool for turning a version 1.0 library into a sqlite3 library.

BiblioSpec components

The BiblioSpec package contains the following programs:

  • BlibBuild creates a library of peptide MS/MS spectra from a variety of different database search results.
  • BlibFilter removes redundant spectra from a library.
  • BlibSearch searches a spectrum library for matches to query spectra, printing the results to a report file.
  • BlibToMS2 writes a library in a text MS2 file format.
  • LibToSqlite3 converts a BiblioSpec 1.0 library to a 2.0 library in sqlite3 format.

Download

BiblioSpec is freely available under the BSD license. Click here to go to the Download and build page.

Several reference libraries will be available soon for download.

More information

An overview of all file formats including a list of all the database search files that can be used to build libraries.




BlibBuild


Description

Creates a library of spectra with known peptide and/or small molecule identifications. Typically, these identifications are done with a database search such as SEQUEST or Mascot, sometimes followed by an evaluation step such as percolator or Peptide Prophet. BlibBuild accepts files from a variety of database search programs, as well as some other spectral library formats. File formats are identified by file extension, which are given in the table below. In many cases, the peptide identification (peptide sequence, charge state and optional score) are in a separate file from the spectrum information. Unless noted, it is assumed that both files will be in the same directory.

Database search Peptide ID file extension Spectrum file extension
*RAW includes vendor formats like RAW, WIFF, .D, etc.
Score Used Notes
Generic SSL .ssl   score column A generic format for encoding spectrum library entries.
ByOnic .mzid .MGF, .mzXML, .mzML AbsLogProb  
Comet/SEQUEST/Percolator .perc.xml, .sqt .cms2, .ms2, .mzXML q-value Percolator v1.17 does not include sequence modification information therefore the .sqt file from the SEQUEST search must be present in the same directory, the directory containing the cms2/ms2 spectrum files, or the current working directory.
DIA-NN .speclib   none No separate spectrum file. In the current implementation, no score is imported from the library, so all spectra are imported.
IDPicker .idpXML .mzXML, .mzML FDR The name(s) of the spectrum file(s) are given in the .idpXML file.
MS Amanda .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* q-value  
MSFragger .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* q-value  
MSGF+ .mzid, .pepXML .mzML, .mzXML, .MGF, RAW* expectation value  
Mascot .dat   expectation value No separate spectrum file.
MaxQuant Andromeda msms.txt + evidence.txt + mqpar.xml + modifications.xml .mzML, .mzXML, .MGF, RAW* PEP It is possible to use peaks embedded in the msms.txt, but external spectra files are preferred because the embedded peaks are charge deconvoluted. mqpar.xml must be located in the grandparent, parent, or same directory. A custom modifications.xml, modifications.local.xml, or modification.xml can be placed in the same directory as the search results (or specified using the -x option).
Morpheus .pep.xml, .pepXML .mzXML, .mzML q-value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory. Spectra are looked up by index, which is calculated using (scan number - 1).
OMSSA .pep.xml, .pepXML .mzXML, .mzML expectation value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
OpenSWATH .tsv   m_score column No separate spectrum file.
PEAKS DB .pep.xml, .pepXML .mzXML, .mzML confidence score The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
PLGS MSe final_fragment.csv   score column There need not be a . before 'final_fragment'..
PRIDE .pride.xml   various No separate spectrum file.
PeptideProphet/iProphet .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* probability score The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
PeptideShaker .mzid .MGF confidence score  
Protein Pilot .group.xml   confidence score No separate spectrum file.
Protein Prospector .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* expectation value  
Proteome Discoverer .msf, .pdResult   q-value No separate spectrum file. Libraries cannot be built from databases that do not contain q-values, unless a cutoff score of 0 is explicitly specified.
Proxl XML .proxl.xml .mzML, .mzXML, .MGF, RAW* q-value  
Scaffold .mzid .MGF, .mzXML, .mzML peptide probability  
Spectronaut .csv   none Spectronaut Assay Library export. No separate spectrum file.
Spectrum Mill .pep.xml, .pepXML .mzXML, .mzML expectation value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
X! Tandem .xtan.xml   expectation value No separate spectrum file.

Usage

BlibBuild [options] <peptide id file>[+] <library name>

Input

  • <peptide id file> – A file containing peptide spectrum matches to be included in the library. The associated spectrum files should be in the same directory as the peptide id file but should not be given on the command line. See the above table for recognized formats. Multiple files may be listed together.
  • <library name> – The name of the library being created. An existing library may be overwriten or added to.

Output

A spectrum library in in sqlite3 format.

Options

  • -o Overwrite existing library. Default append.
  • -S <filename> Read from file as though it were stdin.
  • -s Result file names from stdin. e.g. ls *sqt | BlibBuild -s new.blib.
  • -u Ignore peptides except those with the unmodified sequences from stdin.
  • -U Ignore peptides except those with the modified sequences from stdin.
  • -H Use more than one decimal place when describing mass modifications.
  • -C <file size> Minimum file size required to use caching for .dat files. Specifiy units as B,K,G or M. Default 800M.
  • -c <cutoff> Score threshold (0-1) for PSMs to be included in library. Higher threshold is more exclusive.
  • -v <level> Level of output to stderr (silent, error, status, warn). Default status.
  • -L Write status and warning messages to log file.
  • -m <size> SQLite memory cache size in Megs. Default 250M.
  • -l <level> ZLib compression level (0-?). Default 3.
  • -i <library_id> LSID library ID. Default uses file name.
  • -a <authority> LSID authority. Default proteome.gs.washington.edu.
  • -x <filename> Specify the path of XML modifications file for parsing MaxQuant files.
  • -p <filename> Specify the path of XML parameters file for parsing MaxQuant files.
  • -P <float> Specify pusher interval for Waters final_fragment.csv files.
  • -d [<filename>] Document the .blib format by writing SQLite commands to a file, or stdout if no filename is given.
  • -E Prefer reading peaks from embedded spectra (currently only affects MaxQuant msms.txt)
  • -A Output messages noting ambiguously matched spectra (spectra matched to multiple peptides)
  • -K Keep ambiguously matched spectra
 

 




BlibFilter


Description

Create a library from an existing one such that the new library has only one spectrum for each peptide ion. The representative spectrum is chosen by taking the dot product of all pairs of spectra for a peptide and selecting the one with the highest average score.

Usage

BlibFilter [options] <redundant-library> <filtered-library>

Input

  • <initial library> – A library file with multiple spectra for all or some peptide ions.
  • <output library> – The name to be given to the resulting library.

Output

A library of spectra for the same peptides as the initial library, but with only one spectrum per peptide ion.

Options

  • -m [ --memory-cache ] <size> – SQLite memory cache size in Megs. Default 250M.
  • -n [ --min-peaks ] <num> – Only include spectra with at least this many peaks. Default 20.
  • -s [ --min-score ] <score> – Best spectrum must have at least this average score to be included. Default 0.
  • -p [ --parameter-file ] <file> – File containing search parameters. Command line values override file values.
  • -v [ --verbosity ] <level> – Control the level of output to stderr. (silent, error, status, warn, debug, detail, all) Default status.
  • -h [ --help ]– Print help message.



BlibSearch


Description

Search a spectrum library for matches to query spectra.

Usage

BlibSearch [options] <spectrum filename> <library filename>[+]

Input

  • <spectrum filename> – A file containing spectra to search. File formats accepted are .ms2, .cms2, .mzXML, .mzML, .MGF, and .wiff (Windows only).
  • <library name> – The library to be searched for matches to the query. Libraries may be filtered (the output of BlibFilter) or redundant (the output of BilbBuild). More than one library can be listed on the command line.

Output

Results are printed to a report file (tab-delimited text). The file may be named with the --report-file option or by default it is named after the spectrum file with the extension replaced with .report. A seprate report file is written for any decoy spectra searched. An optional sqlite .psm file may also be produced.

Options

  • -c [ --clear-precursor ] <true|false> – Remove the peaks in a X m/z window around the precursor from the query and library spectrum. Default true.
  • --topPeaksForSearch <num> – Use this many of the highest intensity peaks. Default 100.
  • -w [ --mz-window ] <size> – Compare query to library spectra with precursor m/z +/- size. Default 3.
  • -L [ --low-charge <charge> – ] Search only spectra with charge no less than this. Default 1.
  • -H [ --high-charge ] <charge> – Search only spectra with charge no higher than this. Default 5.
  • -m [ --report-matches ] <num> – Return this number of the best matches for each query. Use -1 to report all. Default 5.
  • --psm-result-file <name> – Return results in a .psm file of the given name. Default no .psm file.
  • -R [ --report-file ] <name> – Return results in report file of the given nam. Default is .report.
  • --preserve-order – Search spectra in the order they appear in the file. Default to search as sorted by precursor m/z.
  • -p [ --parameter-file ] <name> – File containing search parameters. Command line values override file values.
  • -v [ --verbosity ] <level> – Control the level of output to stderr. (silent, error, status, warn, debug, detail, all) Default status.
  • -h [ --help ] – Print help message.



BlibToMS2


Description

Write an MS2 file that contains all spectra in a library.

Usage

BlibToMS2 [options] <library>

Input

  • <library> – a spectrum library file, filtered or redundant.

Output

The spectra are printed to a file named <library>.ms2 in the MS2 format. The scan number is replaced with the library ID number. Two 'D' lines contain the peptide sequence with and without modifications.

Options

  • -f [ --file-name ] <ms2 file>– Use this name for the output MS2 file rather than the default name, <library>.ms2.
  • -m [ --mz-precision ] <num>– Write the peak m/z values with this many digits of precision. Default 2.
  • -i [ --intenisty-precision ] <num>– Write the peak intensity values with this many digits of precision. Default 1.
  • -p [ --parameter-file ] <file> – Specify parameters in a separate file. Command line vales override the file.
  • -v [ --verbose ] <silent|error|status|warn> – Set the verbosity level of the output to stderr. The default level is status.
  • -h [ --help ] – Print the help message.



LibToSqlite3


Description

Converts a BiblioSpec 1.0 library to a 2.0 library in sqlite3 format.

Usage

LibToSqlite3 <old version lib> <new lib name>

Input

  • <old version lib> – A BiblioSpec 1.0 library file.
  • <new lib name> – The name to be given to the converted library.

Output

A spectrum library in in sqlite3 format.




BiblioSpec Supported Formats


Database search Peptide ID file extension Spectrum file extension
*RAW includes vendor formats like RAW, WIFF, .D, etc.
Score Used Notes
Generic SSL .ssl   score column A generic format for encoding spectrum library entries.
ByOnic .mzid .MGF, .mzXML, .mzML AbsLogProb  
Comet/SEQUEST/Percolator .perc.xml, .sqt .cms2, .ms2, .mzXML q-value Percolator v1.17 does not include sequence modification information therefore the .sqt file from the SEQUEST search must be present in the same directory, the directory containing the cms2/ms2 spectrum files, or the current working directory.
DIA-NN .speclib   none No separate spectrum file. In the current implementation, no score is imported from the library, so all spectra are imported.
IDPicker .idpXML .mzXML, .mzML FDR The name(s) of the spectrum file(s) are given in the .idpXML file.
MS Amanda .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* q-value  
MSFragger .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* q-value  
MSGF+ .mzid, .pepXML .mzML, .mzXML, .MGF, RAW* expectation value  
Mascot .dat   expectation value No separate spectrum file.
MaxQuant Andromeda msms.txt + evidence.txt + mqpar.xml + modifications.xml .mzML, .mzXML, .MGF, RAW* PEP It is possible to use peaks embedded in the msms.txt, but external spectra files are preferred because the embedded peaks are charge deconvoluted. mqpar.xml must be located in the grandparent, parent, or same directory. A custom modifications.xml, modifications.local.xml, or modification.xml can be placed in the same directory as the search results (or specified using the -x option).
Morpheus .pep.xml, .pepXML .mzXML, .mzML q-value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory. Spectra are looked up by index, which is calculated using (scan number - 1).
OMSSA .pep.xml, .pepXML .mzXML, .mzML expectation value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
OpenSWATH .tsv   m_score column No separate spectrum file.
PEAKS DB .pep.xml, .pepXML .mzXML, .mzML confidence score The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
PLGS MSe final_fragment.csv   score column There need not be a . before 'final_fragment'..
PRIDE .pride.xml   various No separate spectrum file.
PeptideProphet/iProphet .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* probability score The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
PeptideShaker .mzid .MGF confidence score  
Protein Pilot .group.xml   confidence score No separate spectrum file.
Protein Prospector .pep.xml, .pepXML .mzML, .mzXML, .MGF, RAW* expectation value  
Proteome Discoverer .msf, .pdResult   q-value No separate spectrum file. Libraries cannot be built from databases that do not contain q-values, unless a cutoff score of 0 is explicitly specified.
Proxl XML .proxl.xml .mzML, .mzXML, .MGF, RAW* q-value  
Scaffold .mzid .MGF, .mzXML, .mzML peptide probability  
Spectronaut .csv   none Spectronaut Assay Library export. No separate spectrum file.
Spectrum Mill .pep.xml, .pepXML .mzXML, .mzML expectation value The names of the .mzXML files are given in the .pep.xml file and may be in the parent or grandparent directory.
X! Tandem .xtan.xml   expectation value No separate spectrum file.



BiblioSpec input and output file formats


BiblioSpec makes use of several file formats for input and output. Below are descriptions of these along with links to additional information.

Database search result files

In most cases libraries are built from database search result files. Supported formats are listed on the BlibBuild page

BlibBuild .ssl file

For peptide or small molecule identifications that do not come from one of the supported database searches, BiblioSpec supports a generic tab-delimited text file format refered to as ssl (spectrum sequence list). Here is a small example file. An ssl file must end with the '.ssl' extension and have a header line with the following column names in it (the score-type, score, and retention-time columns are optional):

file	scan	charge	sequence	score-type	score	retention-time

additional columns for small molecule use may be included (the sequence column should be omitted for small molecule libraries - here is a small example file):

adduct	chemicalformula	moleculename	inchikey	otherkeys

additional columns for ion mobility information may be included - css units are square Angstroms, ion mobility units can be 'ms' (drift time msec), 'V' (FAIMS voltage), '1/K0' (inverse K0), or 'none':

ion-mobility	ion-mobility-units	ccs

Each of the following lines contains information for one spectrum. The first column contains a full or relative path to a file containing the spectrum (e.g. vendor formats like .raw, .wiff, etc. or .ms2, .mzML, .mzXML, .mgf). In an .ms2 file there are four types of lines. Lines beginning with 'H' are header lines and contain information about how the data was collected as well as comments. They appear at the beginning of the file. Lines beginning with 'S' are followed by the scan number and the precursor m/z. Lines beginning with 'Z' give the charge state followed by the mass of the ion at that charge state. Lines beginning with 'D' contain information relevant to the preceeding charge state. BlibToMs2's output will include D-lines with the sequence and modified sequence. The file is arranged with these S, Z and D lines for one spectrum followed by a peak list: a pair of values giving each peaks m/z and intensity. Here is an example file.

The second column has an id for that spectrum, typically a scan number or index number. The third column is the charge state of the spectrum. The fourth column contains the peptide sequence, with the addition of any modifications given as a mass shift (the difference between the modified and unmodified residue) following the modified residues. For example,

TASEFDC[+57.0]SAIO[+16.0]AQDK

Peptides with n-terminal modifications should have these mass shift follow the first residue.

The score-type column can be any of the following:

UNKNOWN

PERCOLATOR QVALUE

PEPTIDE PROPHET SOMETHING

SPECTRUM MILL

IDPICKER FDR

MASCOT IONS SCORE

TANDEM EXPECTATION VALUE

PROTEIN PILOT CONFIDENCE

SCAFFOLD SOMETHING

WATERS MSE PEPTIDE SCORE

OMSSA EXPECTATION SCORE

PROTEIN PROSPECTOR EXPECTATION SCORE

SEQUEST XCORR

MAXQUANT SCORE

 

and the score column is a floating point value representing the spectrum's score of that type. The retention time column can be used to specify retention times in minutes; otherwise the values from the spectrum file will be used. Scores fall into three categories: probability that identification is correct, probability that identification is incorrect, or not a probability score. This information can be found in the ScoreTypes table.

Library files

BiblioSpec library files are in the sqlite3 format, usually with a ".blib" filename extension. Each library is a small database that you can search and manipulate with standard SQL commands using, for example, the sqlite3 command line tools or SQLite Expert Personal.

Details on the BiblioSpec SQLite schema can be found here.




Example .ssl file


file	scan	charge	sequence
demo.ms2	8	3	VGAGAPVYLAAVLEYLAAEVLELAGNAAR
demo.ms2	1806	2	LAESITIEQGK
demo.ms2	2572	2	ELAEDGC[+57.0]SGVEVR
demo.ms2	3088	2	TTAGAVEATSEITEGK
demo.ms2	3266	2	DC[+57.0]EEVGADSNEGGEEEGEEC[+57.0]
demo.ms2	9734	3	IWELEFPEEAADFQQQPVNAQ[-17.0]PQN
demo.ms2	20919	3	VHINIVVIGHVDSGK
../elsewhere/spec.mzXML	00497	2	LKEPAQNTADNAK
../elsewhere/spec.mzXML	00680	2	ALEGPGPGEDAAHSENNPPR
../elsewhere/spec.mzXML	00965	2	FFSHEAEQK
../elsewhere/spec.mzXML	01114	2	C[+57.0]GPSQPLK
../elsewhere/spec.mzXML	01382	2	AVHVQVTDAEAGK



Example .ms2 file


H	CreationDate	Mon Apr 12 15:12:14 2010
H	Extractor	BlibToMs2
H	Library	/home/me/research/search/demo.blib
S	1	1	636.34
Z	2	1253.36
D	seq	FKNGFQTGSASK
D	modified seq	FKNGFQTGSASK
187.40	12.5
193.10	19.5
194.30	13.7
198.30	29.8
199.10	12.2
208.30	23.1
208.90	11.4
210.30	11.8
213.00	3.3
214.50	4.3
216.10	32.8
219.10	11.2
221.00	14.3
222.10	64.0
225.10	16.6
226.00	31.6
228.30	7.2
229.10	8.5
230.50	58.2
231.20	236.1
232.20	75.8
233.60	2.4
234.20	51.4
235.10	5.6
236.30	30.2
239.70	14.4
241.30	34.8
242.30	14.2
244.30	9.0
S	2	2	745.3
Z	2	1471.7
D	seq	NFLETVELQVGLK
D	modified seq	NFLETVELQVGLK
1224.60	7.9
1228.70	468.9
1230.40	658.5
1231.50	144.2
1240.00	11.7
1242.70	45.9
1243.80	16.8
1253.80	17.2
1255.00	7.9
1255.80	14.4
1259.70	15.5
1273.10	5.9
1275.90	10.5
1277.10	7.8
1283.30	4.7
1296.50	19.2
1299.50	13.0
1307.40	6.1
1308.40	21.3
1313.00	1.7
1313.80	5.5
1315.40	3.6
1316.80	22.3
1323.90	1.5
1325.50	40.5
1326.30	75.9
S	3	3	732.1
Z	2	1444.7
D	seq	NEVSAMPTLLLFK
D	modified seq	NEVSAMPTLLLFK
209.00	62.5
210.30	12.8
216.00	87.0
220.10	58.0
224.90	4.9
226.10	418.2
227.00	68.3
227.90	46.7
229.20	13.3
231.10	12.7
238.10	209.1
239.20	15.0
244.10	953.8
245.20	90.0
245.90	20.4
252.30	8.8
255.30	38.8
260.20	9.4
262.10	35.0
270.00	10.9
275.80	21.8
277.40	6.3
279.10	12.7
280.20	49.8



Example .ssl file for small molecules


Note that these are tab separated fields, and the otherkeys field itself is tab separated.

file	scan	charge	adduct	inchikey	chemicalformula	moleculename	otherkeys
dexcaf_051017.mzML	01369	-1	[M-H]	ZXPLRDFHBYIQOX-BTBVOZEKSA-N	C24H44O21N0	Glc04Reduced
dexcaf_051017.mzML	01639	-1	[M-H]	NBVGBCYERZIRIP-JAMOUWTMSA-N	C30H54O26N0	Glc05Reduced
dexcaf_051017.mzML	01855	-1	[M-H]	PNHJKLJIDNHXFR-ZGJYWSOBSA-N	C36H64O31N0	Glc06Reduced
dexcaf_051017.mzML	02029	-1	[M-H]	NVKJDLBVRSXYRE-BMFDHOHESA-N	C42H74O36N0	Glc07Reduced
dexcaf_051017.mzML	02179	-1	[M-H]	YMRGEPQWJZHXFF-MGQBKJSVSA-N	C48H84O41N0	Glc08Reduced
dexcaf_051017.mzML	01079	-1	[M-H]	RYYVLZVUVIJVGH-UHFFFAOYSA-N	C8H10N4O2	Caffeine	"InChI:1S/C8H10N4O2/c1-10-4-9-6-5(10)7(13)12(3)8(14)11(6)2/h4H,1-3H3	HMDB:01847	CAS:58-08-2	SMILES:Cn1cnc2n(C)c(=O)n(C)c(=O)c12"



Download and build


Download

The BiblioSpec source code is available as part of the ProteoWizard project on GitHub:

ProteoWizard git repository

A Mascot Parser license should also be requested if you intend to parse Mascot .dat files; once you receive the download instructions:

  • On Windows, the libraries should be installed to C:\Program Files (x86)\Matrix Science\Mascot Parser (32-bit) or C:\Program Files\Matrix Science\Mascot Parser (64-bit)
  • On Linux, the libraries should be installed to /usr/local/msparser/gnu

You will need Visual Studio 2017 to build.

Build

  1. Download (or clone with git) the trunk/pwiz directory from the ProteoWizard git repository.
  2. Open a command prompt (Windows), or a terminal (Linux) and cd to the root of the downloaded source files (e.g. %HOMEDIR%\Documents\pwiz)
  3. Optional: Run clean.bat (Windows) or clean.sh (Linux) to remove files from a previous build.
  4. Run either quickbuild.bat (Windows) or quickbuild.sh (Linux) with the arguments: -j<number of threads to build with> --hash optimization=space address-model=<32|64> pwiz_tools/BiblioSpec > path-to-build-log-file. For example, a 64-bit Windows build using four threads may be done with the command "quickbuild.bat -j4 --hash optimization=space address-model=64 pwiz_tools\BiblioSpec > build.log". Note that "optimization=space" may be omitted for a debug build.

The resulting build will be located in the build-<os>-<architecture>/pwiz_tools/BiblioSpec directory.

Pre-compiled binaries

64 bit Windows binaries (BlibBuild.exe, BlibFilter.exe and BlibToMs2.exe) are available from the ProteoWizard automated build and test system. (If you encounter a "Log in to TeamCity" page, just click the "log in as guest" link to proceed.) Note that this download does not provide a proper installer: it's intended for updating existing installations only. It likely will not function if unzipped to a bare directory.