SRM - PRM Session topics
Day 1 - Monday, October 5, 2020
- Intro to quantitative Analysis
- Intro to Skyline: Hands-on
- Hands-on: Targeted MS/MS (PRM)
- Absolute quantification and calibration curves
- Keynote: HDL proteomics in chronic kidney disease
Day 2 - Tueday, October 6, 2020
Using indexed retention time (iRT)
SRM/PRM instrument set up
Panorama, AutoQC
Tutorial: Manual data analysis
Hands-On: Grouped study data processing with Skyline
Review concepts
DIA - PTM Session Topics
Day 1 - Thursday October 8, 2020
- Welcome and intro
- Tutorial: Analysis of DIA data in Skyline
- DIA/PRM instrument setup
- Peptide detection directly from DIA (Walnut)
- (Keynote)
Day 2 - Friday October 9, 2020
- DIA walkthough
- intro to PTM considerations
- histone PTM walkthroughv
- TBD
- TBD
Small Molecule Session Topics
Day 1 - Thursday October 8, 2020
10am - 6:30 pm (All times EDT)
- Welcome and intro to Skyline for small molecules
- Small molecule method development
- "Futureproofing" metabolomics
- Small molecule method transfer and quantification
- Advanced small molecule quantification
Day 2 - Friday October 9, 2020
9am - 6:30 pm (All times EDT)
- SkyJam (optional) – High resolution metabolomics
- Ion mobility mass spectrometry in proteomics and metabolomics
- Analysis of lipidomics data in Skyline
- System suitability tracking
- Retention time prediction and glycan analysis
- Gas chromatography-mass spectrometry analysis
- The future of small molecule analysis in Skyline