|Don Davis Prior to coming to Vanderbilt and working with Dr. John McLean, Davis worked with Dr. Jorge Albina at Brown University. The Albina lab studies the origin of repair macrophages and how infection alters the healing process. This research is critical because wound repair macrophages produce cytokines (proteins) that promote healing and inflammation. Davis' work analyzed how small molecules (flavonoids) played a role in wound healing using ELISA analytical techniques. Flavonoids have been shown to inhibit lipopolysaccharide (LPS) stimulated pro-inflammatory cytokines (TNF-α and IL-6) from macrophage cell lines in vitro; however, little is known about the effect of flavonoids on wound repair macrophages ex vivo. Results showed fisetin (5 & 10 µM) inhibiting pro-inflammatory cytokines TNF-α and IL-6 in ex vivo macrophages but toxic at 20 µM.
The Development, Validation and Clinical Application of a LC – MS/MS Method for Absolute Quantification of Anti-Epileptic Drugs in SerumRoutine quantification of Anti-Epileptic Drug (AED) small molecules in clinical laboratories is challenging as current methods, such as immunoassays and liquid chromatography - UV detection do not possess either the selectivity, or the low limits of detection and quantification required in clinical applications. This work reports a RPLC – MS/MS method to detect and quantify AEDs in human blood serum. Calibration curves for each AED extracted from serum were generated via Skyline to assess the range of linearity, sensitivity, and other figures of merit (e.g. LOD, LLOQ and ULOQ). Read More
The optimized method described herein provides sample preparation procedures to extract AEDs from blood serum and assesses AED analyte recovery, matrix effects, precision, accuracy, differentiation, stability and carry-over. This method will provide clinical laboratories with a robust and selective quantitative protocol for measuring AEDs in blood serum and clinicians the information necessary to minimize side effects and individually optimize AED medication dosage.