De-isotoping direct infusion lipidomics measured by MRM


Dear Skyline Team,

I would like to perform de-isotoping on my direct infusion lipidomics data from a triple quad MRM experiment. While just performing a correction for M+2 isotopic overlaps based on theoretic abundance of 13C would be straigtforward, the additional effect of distribution of heavy carbons in the detected fragment in Q3 makes the calculation quite a bit more complex. Could I use skyline to perform this de-isotoping routine? Are there any other tools that you could recommend?

Thank you very much in advance for your advice. Best wishes, Thomas