2014 EMBO Course - Targeted Proteomics 

EMBO Practical Course: Targeted proteomics: Experimental design and data analysis 

Conducted in Barcelona, Spain, 28 Sep. – 3 Oct. 2014

Different targeted proteomics strategies have recently emerged in the field of proteomics that enable the detection and quantification of a predetermined subset of proteins with a high degree of sensitivity and reproducibility across many samples. This EMBO Practical Course aimed to fill in the gap between theory and the actual implementation of targeted proteomics workflow. By the end of the week, students would have the necessary know-how to implement targeted proteomics workflows in their own research laboratories. 

Below are vidoe-taped recordings of the lectures and demonstrations delivered during the course.  The sequence of presentations follows that of the program agenda. Enjoy! 

 


 Day 1: Biological significance of targeted proteomics experiments

 

 

Dr. Ruedi Eebersold

Keynote session
"Design and Biological Significance of Targeted Proteomic Experiments” with Dr. Ruedi Aebersold (ETH).

 




 

Dr. Eduard Sabidó

Tutorial/practical demonstration
Introduction to Targeted Proteomics Workflows” with Dr. Eduard Sabidó (CRG).

 


 

Brendan MacLean

Tutorial/practical demonstration
Skyline: Software Suite for Targeted Proteomics” with Brendan MacLean (Univ. of Washington).

 


Day 2: How to get optimal targeted proteomics assays?

 

Dr. Cristina Chiva

Tutorial/practical demonstration
Instrument and Method Parameter Optimization for Targeted Proteomics” with Dr. Cristina Chiva (CRG).

 



 


 

Dr. Olga Schubert

Tutorial/Practical demonstration
Scheduled Targeted Methods and iRT Standards” with Dr. Olga Schubert (ETH).

 


 Day 3: Data interpretation and statistical analysis

 Prof. Olga Vitek

 

Keynote session
Experimental Design and Statistics for Targeted Proteomics” with Prof. Olga Vitek (Northeastern University).

 

 


Meena Choi

Tutorial/Practical demonstrations
Statistical Analysis with MSstats” with Meena Choi (Purdue University).

 


 


 

Dr. Christina Ludwig

Tutorial/Practical demonstration
"
Absolute Quantitation in Targeted Proteomics Studies" with Dr. Christina Ludwig (ETH).

 



Day 4: Turning targeted proteomics high-throughput I

Dr. Ludovic Gillet

Keynote session
Targeted Data Extraction of the MS/MS Spectra Generated by Data-Independent Acquisition: a new concept for consistent and accurate proteome analysis”  with Dr. Ludovic Gillet (ETH).

 

 

 


 

Ariel Bensimon

Tutorial/Practical demonstration
Monitoring Phosphorylations by Targeted Proteomics” with Ariel Bensimon (ETH).

 



 

Dr. Ludovic Gillet

Tutorial/Practical demonstration
Generation of Libraries for the Identification in Mass Specotmetry Data-Independent Datasets” with Dr. Ludovic Gillet (ETH).

 


Dr. Olga Schubert

Keynote session
The Quantitative Proteome of Mycobacterium Tuberculosis by Targeted Mass Spectrometry” with Dr. Olga Schubert (ETH).

 


Day 5: Turning targeted proteomics high-throughput II 

Prof. Michael MacCoss

Keynote session
Design and Biological Significance of Targeted Proteomics Experiments: PRM and MSX”  with Prof. Michael MacCoss (Univ. of Washington).

 

 


 


Pages