Issue 721: Skyline cannot properly analyze Agilent QQQ with waste diverter in the beginning of the run

issues
Status:open
Assigned To:Brendan MacLean
Type:Defect
Area:Skyline
Priority:3
Milestone: 
Opened:2020-03-26 by ayson
Changed:2020-03-26 by ayson
Resolved:
Resolution:
Closed:
2020-03-26 ayson
Title»Skyline cannot properly analyze Agilent QQQ with waste diverter in the beginning of the run
Assigned ToGuest»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
HI Brendan,

We have acquired HILIC data in Agilent QQQ with diverter valve to waste from 0-0.2 mins. From 0.2 to 5 mins, mass spec was turned on. When I analyze the data in Skyline, I am only seeing the chromatogram from 0-0.2 mins but never 0.2 to 5 mins. The data looks fine in Masshunter Quant but not in skyline. I have sent you more detailed report in the attached word document.


It is vital that skyline can process these types of data because more small molecules will be diverter their initial flow to waste to protect mass spec instrument. Thank you so much!!!

Best regards,
Marites
 
 032620_skyline_not properly analyzing Agilent QQQ data with waste diverter on.docx