Issue 695: Failed importing results DIA

issues
Status:resolved
Assigned To:Brendan MacLean
Type:Defect
Area:Skyline
Priority:3
Milestone: 
Opened:2019-10-29 by an.staes
Changed:2019-11-09 by Brendan MacLean
Resolved:2019-11-09 by Brendan MacLean
Resolution:Fixed
Closed:
2019-10-29 an.staes
Title»Failed importing results DIA
Assigned ToGuest»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
Dear,

I'm importing results file for DIA through command line, but don't get any further than 40%. I'm using skyline Daily 19.1.1.283; import mzML file.
Any idea what is wrong?
The following error appears:
Error: Failed importing the results file H08127_uPAC_CMB-233_DIA_Plasma_Depleted_Rep-1.mzML.
[2019/10/25 17:48:57]    Message: System.IO.IOException: Error: Failed importing the results file Importing H08127_uPAC_CMB-233_DIA_Plasma_Depleted_Rep-1.mzML.
Message: pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file 'C:\An\Skyline\Data\H08127_uPAC_CMB-233_DIA_Plasma_Depleted_Rep-1.mzML'.
Win32 Error: 80 ---> System.IO.IOException: Win32 Error: 80 ---> System.IO.IOException: Win32 Error: 80
   at pwiz.Skyline.Util.FileStreamManager.GetTempFileName(String basePath, String prefix, UInt32 unique) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Util\UtilIO.cs:line 788
   at pwiz.Skyline.Util.FileStreamManager.GetTempFileName(String basePath, String prefix) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Util\UtilIO.cs:line 760
   at pwiz.Skyline.Model.Results.ChromGroups.SpillFile.<>c__DisplayClass9_0.<CreateFileStream>b__0() in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 700
   at pwiz.Skyline.Util.Helpers.Try[TEx](Action action, Int32 loopCount, Int32 milliseconds) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Util\Util.cs:line 1869
   at pwiz.Skyline.Model.Results.ChromGroups.SpillFile.CreateFileStream(String cachePath) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 698
   at pwiz.Skyline.Model.Results.ChromGroups.GetFileStream(Int32 chromIndex) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 577
   at pwiz.Skyline.Model.Results.BlockWriter.WriteBlock(Int32 chromatogramIndex, IBlockedList blockedList) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 443
   at pwiz.Skyline.Model.Results.BlockedList`1.Add(Int32 chromatogramIndex, TData data, BlockWriter writer) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 202
   at pwiz.Skyline.Model.Results.ChromCollector.AddPoint(Int32 chromatogramIndex, Single intensity, Nullable`1 massError, BlockWriter writer) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\ChromCollector.cs:line 78
   at pwiz.Skyline.Model.Results.ChromDataCollectorSet.ProcessExtractedSpectrum(Single time, Collectors chromatograms, Int32 scanId, ExtractedSpectrum spectrum, Action`2 addCollector) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1476
   at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ProcessSpectrumList(MsDataSpectrum[] spectra, ChromDataCollectorSet chromMap, Single rt, SpectrumFilter filter, String scanId) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 497
   at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatogramsLocked() in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 331
   at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatograms() in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 217
   at pwiz.Skyline.Model.Results.SpectraChromDataProvider.<SetRequestOrder>b__46_0() in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 561
   --- End of inner exception stack trace ---

Thank you for looking into this.

Kind regards,
An

2019-11-09 Brendan MacLean
resolve as Fixed
Statusopen»resolved
Please try this again with the current Skyline-daily. If it fails, you should at least get a better error, but it may also be fixed.

2019-11-09 Brendan MacLean
What is the source of your DIA data? What was the raw data type before you converted to mzML and how long a gradient was measured?