Issue 571: issue with normalization ratio

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:1
Milestone:4.1
Opened:2018-04-23 by jfidelin
Changed:2018-10-01 by Brian Pratt
Resolved:2018-10-01 by Brian Pratt
Resolution:Fixed
Closed:2018-10-01 by Brian Pratt
2018-04-23 jfidelin
Title»issue with normalization ratio
Assigned ToGuest»Brendan MacLean
Type»Defect
Area»Skyline
Priority»1
Milestone»4.1
Skyline Daily v. 4.1.1.11903, Instrument: Thermo QE+.
Problem:
For all our metabolites (analyzed using polarity switch), we have defined a normalization method: Ratio to surrogate. The ‘normalizer’ is a heavy labelled amino acid.
When working with many samples (40) we have observed that for ONLY some metabolites NO normalized ratio are reported.
We believe we see the following pattern: normalized values are not available for metabolites that only appear in one polarity mode. The Ratio to surrogate is measured in both positive and negative modes.
Using only one raw file (one sample), we cannot reproduce the problem.

We have attached an .csv file with an data example: Look for '02_carnitine' (line #2148 to #2221) as an example of missing normalization but signals in positive mode.

Will be glad to share .sky file

Very best, Justine and henrik
 
 Polar hm full.csv

2018-04-23 Brendan MacLean
Assigned ToBrendan MacLean»Brian Connolly

2018-04-23 Brendan MacLean
Assigned ToBrian Connolly»Brian Pratt

2018-10-01 Brian Pratt
In the end this was handled on the support board. See https://skyline.ms/announcements/home/support/thread.view?rowId=36302

2018-10-01 Brian Pratt
resolve as Fixed
Statusopen»resolved
Assigned ToGuest»Brian Pratt
In the end this was handled on the support board. See https://skyline.ms/announcements/home/support/thread.view?rowId=36302

2018-10-01 Brian Pratt
close
Statusresolved»closed
Assigned ToBrian Pratt»Guest