Issue 539: Heavy/Light Peptide Chromatogram Import Range Discrepancies

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:3
Milestone:4.1
Opened:2017-09-16 by robwsprung
Changed:2017-10-18 by Brendan MacLean
Resolved:2017-10-18 by Brendan MacLean
Resolution:Fixed
Closed:2017-10-18 by Brendan MacLean
2017-09-16 robwsprung
Title»Heavy/Light Peptide Chromatogram Import Range Discrepancies
Assigned ToGuest»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
Milestone»4.1
I have imported a Thermo RAW DDA file in order to setup a scheduled PRM run. I have the Full-Scan settings to import all matching scans and "Synchronize Zooming" is also selected. I find that there are many cases where different retention time ranges are imported for heavy and light peptides, in some cases resulting in the peak of interest being absent in one of the channels (see attached /Powerpoint slides).

Is there a setting or a conflict I may be overlooking? Thanks.

Robert

2017-09-16 Brendan MacLean
resolve as Not Repro
Statusopen»resolved
Assigned ToGuest»Brendan MacLean
Not seeing the PowerPoint slides. For PRM this would usually indicate a problem scheduling the light and heavy channels over the same time intervals. Did Skyline generate the instrument method?

2017-10-18 Brendan MacLean
reopen
Statusresolved»open
Okay, we got your example files.

2017-10-18 Brendan MacLean
resolve as Fixed
Statusopen»resolved
Assigned ToGuest»Brendan MacLean
This should be fixed in the latest Skyline-daily release which forces the same extraction ranges between multiple precursors of the same peptide/molecule.

2017-10-18 Brendan MacLean
close
Statusresolved»closed
Assigned ToBrendan MacLean»Guest