Issue 509: remoove limit of 200aa or increase this number for analysis top-down or middle-down data

issues
Status:closed
Assigned To:Guest
Type:Todo
Area:Skyline
Priority:3
Milestone:3.7
Opened:2017-05-03 by j sikora
Changed:2018-10-15 by Brendan MacLean
Resolved:2018-10-15 by Brendan MacLean
Resolution:Won't Fix
Closed:2018-10-15 by Brendan MacLean
2017-05-03 j sikora
Title»remoove limit of 200aa or increase this number for analysis top-down or middle-down data
Assigned To»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
Milestone»3.7
Hi Brendan,
I was wondering if it is possible to use Skyline to analyze TopDown data. I found the limit is 200 amino acids for your peptide chain. Is it possible to increase this number? Also it would be interesting to have possibility to analyze not digested proteins. Usually we analyzing proteins up to 30kDa.
Thanks you for any help.
Best
Jacek

2017-05-03 Brendan MacLean
TypeDefect»Todo
I think the number you are talking about is the maximum "peptide length" for applying a protease enzyme, but it sounds like you want to work with entire proteins without digestion at all. Do I have that right? Or are you wanting to measure very large peptides with some very limited protease cleavage that produces very large peptides? Is there any systematic cleavage rule being applied in your case?

I am able to add entire proteins as peptides using Edit > Insert > Peptides. I just did this for MAPK1. Worked great.

2018-10-15 Brendan MacLean
resolve as Won't Fix
Statusopen»resolved
Assigned ToGuest»Brendan MacLean
Since no response, I think we are just going to stick with Edit > Insert > Peptides as the solution here.

2018-10-15 Brendan MacLean
close
Statusresolved»closed
Assigned ToBrendan MacLean»Guest