|Title||»||Improve BlibBuild handling of multiple searches on the same raw data files|
One group reported needing to run two separate searches on every DDA raw data file with a different cleavage enzyme and potential variable modifications in each search. When they go to build a library out of these runs in BlibBuild, it creates a new row for each search for each raw data file, i.e.
File1 - from Search1
File2 - from Search1
File3 - from Search1
File1 - from Search2
File2 - from Search2
File3 - from Search2
The problem with this is that then, because the iRT peptides are only found with Search1, Skyline fails to calibrate iRT values for all of the peptides found with Search2, thinking these files contained no iRT peptides.
If we make BlibBuild smart enough to produce only 1 row per raw data file, that should fix this problem and allow them to get iRT values for all of their detected peptides.