|Title||»||background database with two enzymes doesn't work for first enzyme|
So I just took my human canonical background database which was preprocessed for tryptic peptides. I was doing an experiment with chymotrypsin so it then added those peptides to the database. Unfortunately the autocomplete completely stopped working after that .... maybe the database is too big? Also, while I could add chymotrypsin peptides into the insert > peptides dialog and it would map them to proteins nicely, it would no longer do that for tryptic peptides. Does the background database only work for one enzyme at a time? And if so why doesn't it regenerate the tryptic peptides if I switch the settings back to using trypsin?
I does only work for the active protease in your document. Switching back to a previously calculated digestion should not require digestion again, that information should just be in the background proteome database. So, everything but the enzyme not working when you switch back is expected.
I am going to push this until after v0.6 which is going out in the next few days, and this is not worth the potential destabilization.
Nick, could you please take a look at this. It has been 5 months.
I guess this one is still around...