Issue 453: Add check box to Digestion tab to allow removal of protein N-terminal Methionine

Status:open
Assigned To:Brian Pratt
Type:Todo
Area:Skyline
Priority:3
Milestone:19.2
Opened:2016-02-06 by Brendan MacLean
Changed:2019-06-14 by Brian Pratt
Resolved:
Resolution:
Closed:
2016-02-06 Brendan MacLean
Title»Add check box to Digestion tab to allow removal of protein N-terminal Methionine
Assigned To»bspratt
Type»Todo
Area»Skyline
Priority»3
Milestone»3.6
People have asked a number of times over the years for Skyline to remove the protein N-terminal Methionine from the protein N-terminal peptide. Most recently in this support request:

https://skyline.gs.washington.edu/labkey/announcements/home/support/thread.view?rowId=24953

Since you are currently working in the Peptide Settings - Digestion tab, maybe you could find a way to add a checkbox near the Enzyme dropdown list that causes Skyline to perform this removal when checked. It would have to impact the background proteome as well as PeptideGroup.GetPeptides().

2019-04-23 Brendan MacLean
Milestone3.6»4.3
Requested again by a new user. I still think the ckeckbox on the Digestion tab is the right idea. Not higher priority than your current work, but a little higher priority with a second request. Wish we had more time from interns that could handle something like this.

2019-06-14 Brian Pratt
Milestone4.3»19.2