|Title||»||Skyline should warn when importing a transition list with values that exceed the current instrument m/z range|
Jimmy got tripped up by this small molecule exploration - "1500.7005" was just outside the default range of 1500:
I'm using Skyline for maybe the third time in my life, sitting here with Vagisha figuring out how to get some data into it using the small molecule transition list support. Anyways, things seem to be working well except for one weird case that I hope you can help with.
I have a Q Exactive PRM run. For my test (for data from the Bruce lab down here), I'm trying to use Skyline to quantify this targeted data.
transitions: 1443.6791, 1500.7005, 1098.0482, 1126.5589
I can enter these masses into the small molecule transition list editor (making up charge states as I don't know what they are right now) and Skyline will load the data and correctly highlight the co-eluting peak. The problem is with the 1500.7005 transition where no chromatogram is extracted and I don't understand why. When I extract the data for this transition myself with my own tools, I see a pretty clean signal for this transition's chromatogram. Also when extracted the chromatograms in Xcalibur, the transition showed up too. Can you take a look at this when you get the chance and see if you can figure out why I'm not seeing this 924.70209->1500.7005 transition in Skyilne? Let me know what else you need besides the raw file which is at: