Issue 265: Skyline won't recognize precursor transitions in certain RAW files

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:3
Milestone:2.1
Opened:2013-09-26 by ScottGoulding
Changed:2013-10-18 by Brendan MacLean
Resolved:2013-09-28 by ScottGoulding
Resolution:Fixed
Closed:2013-10-18 by Brendan MacLean
2013-09-26 ScottGoulding
Title»Skyline won't recognize precursor transitions in certain RAW files
Assigned To»goulding@pitt.edu
Type»Defect
Area»Skyline
Priority»3
Milestone»2.1
Hey Brendan,

I'm running a targeted method on our Orbi Elite, but for some reason Skyline isn't recognizing precursor transitions in certain files. I've attached a screen shot of the method used for both runs, a screen shot comparing one peptide where Skyline is and isn't recognizing the precursors, and I've also attached the shared Skyline document. Do you have any idea what is going on?

Thanks,

Scott Goulding
 
 peaks-in-skyline.png
 instrument-method.png
 SkylineQC-Template.zip

2013-09-28 ScottGoulding
resolve as Fixed
Statusopen»resolved
This turned out to be an instrument issue--not a Skyline issue.

2013-10-18 Brendan MacLean
close
Statusresolved»closed
Assigned Togoulding@pitt.edu»Guest