|Title||»||Importing a Document with the Same Replicate, Different Peptides|
|Assigned To||»||Brendan MacLean|
I have two documents, 32DaDIA_autorefined.sky and 32DaDIA_1_autorefined.sky . They contain two separate sets of proteins, but I haven't checked to see if they have overlapping peptides. I imported the same file into both documents called 20120114_CS_Mouse_Embryonic_DIA_130...raw .
Next, I open up 32DaDIA_autorefined.sky and go to File --> Import --> Document and select 32DaDIA_1_autorefined.sky. On the dialog I choose "Merge with existing results by replicate name", and "Merge matching peptides" .
At this point everything went according to plan, the proteins from 1_autorefined.sky appear in the target list of autorefined.sky. But after the merge, the peptides under MybBP1A don't show results in autorefined.sky, when there are results in 1_autorefined.sky . Even stranger is the first peptide under ACTA1; ACTA2; ACTB; ACTBL2; ACTC1; ACTG1; ACTG2
has a few transitions, but not all of them from the original.
The .sky/.skyd files are in /net/maccoss/vol5/home/jegertso/MacCoss/Experiments/20120114_CS_Mouse_Embryonic
.raw file is in /net/maccoss/vol8/raw_data/Q-Exactive/Jarrett/20120114_CS_Mouse_Embryonic