Issue 217: Importing a Document with the DIA analysis of same files, different peptides

issues
Status:open
Assigned To:Brendan MacLean
Type:Defect
Area:Skyline
Priority:3
Milestone:4.3
Opened:2013-01-29 14:14 by Jarrett Egertson
Changed:2018-10-15 10:26 by Brendan MacLean
Resolved:
Resolution:
Closed:
2013-01-29 14:14 Jarrett Egertson
Title»Importing a Document with the Same Replicate, Different Peptides
Assigned To»Brendan MacLean
Type»Defect
Area»Skyline
Priority»3
Milestone»1.4
I have two documents, 32DaDIA_autorefined.sky and 32DaDIA_1_autorefined.sky . They contain two separate sets of proteins, but I haven't checked to see if they have overlapping peptides. I imported the same file into both documents called 20120114_CS_Mouse_Embryonic_DIA_130...raw .

Next, I open up 32DaDIA_autorefined.sky and go to File --> Import --> Document and select 32DaDIA_1_autorefined.sky. On the dialog I choose "Merge with existing results by replicate name", and "Merge matching peptides" .

At this point everything went according to plan, the proteins from 1_autorefined.sky appear in the target list of autorefined.sky. But after the merge, the peptides under MybBP1A don't show results in autorefined.sky, when there are results in 1_autorefined.sky . Even stranger is the first peptide under ACTA1; ACTA2; ACTB; ACTBL2; ACTC1; ACTG1; ACTG2
has a few transitions, but not all of them from the original.

The .sky/.skyd files are in /net/maccoss/vol5/home/jegertso/MacCoss/Experiments/20120114_CS_Mouse_Embryonic

.raw file is in /net/maccoss/vol8/raw_data/Q-Exactive/Jarrett/20120114_CS_Mouse_Embryonic

2014-01-08 08:09 Brendan MacLean
TitleImporting a Document with the Same Replicate, Different Peptides»Importing a Document with the DIA analysis of same files, different peptides
Milestone1.4»2.6
Fix File > Import > Document to work for multiple DIA documents analyzing the same file with different peptides.

2014-08-28 14:29 Brendan MacLean
Milestone2.6»3.1
Didn't make v2.6

2018-10-15 10:26 Brendan MacLean
Milestone3.1»