Issue 202: Improve multiplexing detector to reject multi-precursor isolations that are not really multiplexed

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:3
Milestone:2.1
Opened:2012-10-05 by Brendan MacLean
Changed:2013-04-02 by Brendan MacLean
Resolved:2013-04-02 by Brendan MacLean
Resolution:Fixed
Closed:2013-04-02 by Brendan MacLean
2012-10-05 Brendan MacLean
Title»Improve multiplexing detector to reject multi-precursor isolations that are not really multiplexed
Assigned To»Jarrett Egertson
Type»Defect
Area»Skyline
Priority»3
Milestone»2.1
One Skyline user noted:

I'm trying to use Skyline to quantify what I am calling pseudoswath data obtained on a QExactive instrument.

The acq method goes like this:

1. Full scan m/z 500-890.
2. MS/MS 3 plex msx at 505,515,525 m/z with 10 m/z isolation width on each (starting m/z of scan is 350)
3. MS/MS 3 plex msx at 535,545,555 m/z with 10 m/z isolation width on each (starting m/z of scan is 350)
...etc...
14. MS/MS 3 plex msx at 865,875,885 m/z with 10 m/z isolation width on each (starting m/z of scan is 350)

I have put a skyline doc with a single peptide in it, monitoring 5 transitions. As you can see from the XCalibur screencap, all 5 are present at around 37 minutes from the 715,725,735 msx scan MS/MS scan.

I have tried a bunch of MS/MS DIA isolation schemes, but I can't seem to get it to work (see included .sky), meaning get the transition chromatograms to appear.

>>>> The most likely reason is that the multiplexing detection code is deciding that this is multiplexed data, but then failing to demultiplex it.

2013-04-02 Brendan MacLean
resolve as Fixed
Statusopen»resolved
Assigned ToJarrett Egertson»Brendan MacLean
Jarrett has committed a fix for this.

2013-04-02 Brendan MacLean
close
Statusresolved»closed
Assigned ToBrendan MacLean»Guest