|Title||»||N- and C-terminal protease combinations|
|Assigned To||»||Brendan MacLean|
I did a double enzyme digestion with both trypsin (cleaves c-terminal) and ASP-N (cleaves n-terminal) on some samples that I'd like to look at in Skyline. However, it appears to me that for each 'enzyme' in Skyline you can only specify either C-terminal or N-terminal. Could you tell me how to set up a digestion for Trypsin+ASPN where I specify C-terminal to K/R and N-terminal to D/E?