Issue 185: N- and C-terminal protease combinations

issues
Status:closed
Assigned To:Guest
Type:Todo
Area:Skyline
Priority:4
Milestone:2.1
Opened:2012-08-27 by Jason Held
Changed:2013-04-02 by Brendan MacLean
Resolved:2013-04-02 by Brendan MacLean
Resolution:Fixed
Closed:2013-04-02 by Brendan MacLean
2012-08-27 Jason Held
Title»N- and C-terminal protease combinations
Assigned To»Brendan MacLean
Type»Todo
Area»Skyline
Priority»3
Milestone»1.2
Hi Brendan,

I did a double enzyme digestion with both trypsin (cleaves c-terminal) and ASP-N (cleaves n-terminal) on some samples that I'd like to look at in Skyline. However, it appears to me that for each 'enzyme' in Skyline you can only specify either C-terminal or N-terminal. Could you tell me how to set up a digestion for Trypsin+ASPN where I specify C-terminal to K/R and N-terminal to D/E?

Cheers,
Jason

2012-09-11 Brendan MacLean
Priority3»4
Milestone1.2»2.1

2012-09-11 Brendan MacLean
Maybe the solution is to make the Digestion Enzyme field a check list so that you can select more than one protease at a time, rather than being forced to define protease mixtures in terms of a single element in the Enzyme list. Would that work for you, Jason? Seems to me like it would simplify things a bit.

2012-09-11 Jason Held
Yeah, that would work.

2013-04-02 Brendan MacLean
resolve as Fixed
Statusopen»resolved
Assigned ToBrendan MacLean»Jason Held
I made it possible to specify multi-directional single enzymes instead, because making enzyme choice multiple selection proved too complicated for the time I had available to support this.

2013-04-02 Brendan MacLean
close
Statusresolved»closed
Assigned ToJason Held»Guest