|Title||»||Implement "decrease in old protein" method of calculating half-life|
As we discussed, I'd like to test whether Topograph can measure protein degradation more directly. The method would be something like this:
Calculate % new as usual.
Compare % new to raw % D3.
Assuming that the difference between those two numbers reflects protein that is new but unlabeled, calculate the percentage of the unlabeled protein that is actually new, and subtract it from total unlabeled protein.
Use the resulting figure, "% old," instead of % new, in the log plots to calculate half-life.
Create an option for this method in the drop-down on the HalfLivesForm.