Issue 131: absolute quantification of mTRAQ-modified peptides

issues
Status:closed
Assigned To:Guest
Type:Defect
Area:Skyline
Priority:3
Milestone:1.2
Opened:2011-12-16 by wuxian2010
Changed:2017-10-18 by Brendan MacLean
Resolved:2011-12-16 by Nick Shulman
Resolution:By Design
Closed:2017-10-18 by Brendan MacLean
2011-12-16 wuxian2010
Title»absolute quantification of mTRAQ-modified peptides
Assigned To»nicksh@u.washington.edu
Type»Defect
Area»Skyline
Priority»3
Milestone»1.2
Hi Nick,

I have done a absolute MRM experiment which IS peptides labeled mtraq-04(144Da) and the target peptides labeled mtraq08(149Da).
How shoud I configure the skyline?

Best regards!

WuXian

2011-12-16 Nick Shulman
resolve as By Design
Statusopen»resolved
Assigned Tonicksh@u.washington.edu»wuxian2010@sina.com.cn
You can tell Skyline about the modifications your experiment is using in the "Peptide Settings" dialog:
Settings|Peptide Settings...|Modifications|Edit list...|Add

If you start to type "mtraq" in the "Name" box on that dialog, Skyline will suggest a couple of pre-defined modifications that it knows about.

If you have any more questions about Skyline you should post them on the "Support Board" at:
https://skyline.gs.washington.edu/labkey/project/home/support/begin.view

2017-10-18 Brendan MacLean
close
Statusresolved»closed
Assigned Towuxian2010@sina.com.cn»Guest